Lipoprotein(a) (Lp(a)) ELISA kit: For Research Use Only
Size: 1 x 96 wells Sensitivity: 3.0 µg/dL Dynamic Range: 3 - 405µg/dL Incubation Time: 2.5 hours Sample Type: Serum, Plasma Sample Size: 10 µL Product Developed and Manufactured in the USA
Intended Use The Eagle Biosciences Lipoprotein(a) (Lp(a)) ELISA assay kit is intended for the quantitative determination of Lp(a) in plasma by enzyme linked immunoassay (ELISA). The Lipoprotein(a) (Lp(a)) ELISA assay kit is for research use only and not to be used in diagnostic procedures.
Assay Principle The Lipoprotein(a) (Lp(a)) ELISA assay kit determines total human Lp(a) according to the “sandwich” principle. Lp(a) in samples and standards binds to antibodies which are coated to the microtiter plate. After a washing step a peroxidase labeled detection antibody is added. A second washing step is followed by the addition of the substrate which is converted to a colored product by the peroxidase. The reaction is terminated by the addition of an acidic stop solution. The optical densities are read at 450 nm in a microtiter plate reader. The Lp(a) concentration can be calculated from the standard curve.
Add 100uL of diluted specimen or standard to each microwell
Incubate at room temperature for 60 minutes
Decant and wash each microwell five times with wash buffer (dilute buffer 1:15 with reagent grade water)
Add 100uL of anti-human Apo B-100 conjugate to each well
Incubate at room temperature for 60 minutes
Decant and wash as in step 3
Add 100uL of TMB/peroxide substrate and incubate at room temperature for 30 minutes
Terminate the reaction with 100uL of 0.5N sulfuric acid
Typical Standard Curve
Assay Background Lp(a), unlike Apo AI or Apo B, whose levels vary as a result of diet, exercise, etc. is predominantly a genetic trait whose level remains more or less constant after puberty. More than 13 phenotypes of Lp(a) have been identified having molecular weight of 300-800 Kd. It is bound to both HDL and LDL. Lp(a) interferes with plasminogen, the clot dissolving enzyme, which binds to the arterial endothelial lining. This in turn contributes to blood clot formation, and over a prolonged period of time would lead to significant damage to the coronary arteries. Levels of greater than 30mg/dl have been demonstrated to independently increase the risk of CHD by six fold.
